Real Madrid forward Kylian Mbappe said Benfica's Gianluca Prestianni should be banned from the Champions League after the Argentine was accused of directing a racist slur at Vinicius Jr.
Benfica will also be without head coach Jose Mourinho, who is suspended after receiving a red card in the first leg.
UEFA has provisionally suspended Benfica's Gianluca Prestianni for one match following allegations of a racist slur directed at Real Madrid's Vinicius Jr. during a Champions League match.
UEFA has fined Benfica 40,000 euros for racist behaviour by supporters during their Champions League match against Real Madrid.
Vinicius Jr scored a sublime second-half winner as Real Madrid beat Benfica 1-0 in their Champions League playoff first leg on Tuesday, which was halted for 11 match under FIFA's anti-racism protocol.
Real Madrid overcame an early deficit to defeat Benfica 2-1, securing a 3-1 aggregate victory and a place in the Champions League last 16.
Extra time goals see Chelsea down 10-man Benfica to advance to quarters