Australian Stosur, who has a history of suffering attacks of nerves in front of home fans, struggled to serve out the match at Rod Laver Arena and double-faulted on match point.
Veteran Leander Paes and his partner Samantha Stosur suffered a second round defeat in the mixed doubles event, ending India's campaign at the Australian Open.
Stosur recently announced this month that her partner Liz Astling gave birth to daughter Evie in June.
Stosur won the 2011 US Open, and has been as high as No. 4 in singles. In doubles, Stosur has been ranked No. 1 and won three Grand Slam trophies, most recently at the 2019 Australian Open.
Krawczyk and Skupski became the first pair to successfully defend the mixed doubles title at the All England Club since Czech brother-sister duo Cyril Suk and Helena Sukova in 1997.
Fifteenth seeds Samantha Stosur and Nenad Zimonjic claimed the mixed doubles title with a 6-4, 6-2 win against 14th seeds Chan Hao-Ching of Taiwan and Max Mirnyi of Belarus at Wimbledon on Sunday in the final match of the 2014 championships.
India's challenge in mixed doubles at the Wimbledon Championships ended with second round defeats of Divij Sharan and Rohan Bopanna, on Saturday.
Serena Williams overcame a gritty challenge from unseeded Dutchwoman Kiki Bertens, winning 7-6(7), 6-4, to reach the French Open final, in Paris, on Friday. She will meet Garbine Muguruza, who reached her second Grand Slam final when she beat Australia's Samantha Stosur 6-2, 6-4 in the first semi-final at Roland Garros.
German veteran Tommy Haas provided a reminder of his claycourt skills to beat Stanislas Wawrinka on Thursday and reach the quarter-finals of the Rome Masters where Rafael Nadal and Andy Murray will resume their rivalry.
India's ace tennis star Sania Mirza and her partner Shuai Zhang were knocked out of the women's doubles event of the Luxembourg Open.
World No 1 Serena Williams won a tense battle of U.S. Open champions by beating Australia's Samantha Stosur 7-6(7), 7-6(7) on Wednesday to reach the third round of the Western and Southern Open in Cincinnati.
In a bizarre coincidence, top seed Victoria Azarenka and seventh-seeded Australian Samantha Stosur withdrew from their quarter-final matches at the BNP Paribas Open on Thursday due to leg injuries.
Grand Slam winners Samantha Stosur and Petra Kvitova strolled into the second round of the Dubai championships with straight sets wins on Tuesday after Australian Open champion Victoria Azarenka withdrew due to a foot injury.
Czech Petra Kvitova defeated titleholder Agnieszka Radwanska 6-2, 6-4 in the Dubai Championships on Thursday to set up a semi-final against Caroline Wozniacki.
Bernard Tomic and Lleyton Hewitt gave local fans some excitement before the Australian Open with victories in warm-up tournaments on Saturday while David Ferrer entered the record books.
Sania Mirza and her American partner Bethanie Mattek-Sands scored a straight set win over Francesca Schiavone and Samantha Stosur to move to the quarter-finals of the WTA Premier event in Rome.
Britain's Laura Robson and Spain's Carla Suarez Navarro conjured up big first-round upset victories at the Madrid Open when they surprised fourth seed Agnieszka Radwanska of Poland and ninth-seeded Australian Samantha Stosur on Monday.
World number one Victoria Azarenka pulled out of the Pan Pacific Open citing chronic fatigue while Maria Sharapova was stunned by Samantha Stosur as the event lost its top two seeds on Thursday.
Maria Sharapova continued her untroubled progress at the Qatar Open on Friday, brushing aside Australia's Samantha Stosur 6-2, 6-4 to reach the semi-finals.
Olympic champion Andy Murray looked to build on his recent success by cruising past Alex Bogomolov Jr. and leading a parade of favourites into the second round of the US Open on Monday.
Sania Mirza and Bob Bryan began their partnership with a convincing straight-set victory over Australian pair of Samantha Stosur and Luke Saville in the mixed doubles first round of the Australian Open on Friday.
Samantha Stosur hopes to banish her home court demons on the way to more Grand Slam glory after failing to add to her major trophies in 2012, the Australian said on Friday.
Denmark's Caroline Wozniacki claimed her 20th career title by beating top seed Samantha Stosur 6-2, 4-6, 7-5 in a thrilling Kremlin Cup tennis final on Sunday.
Top seed Samantha Stosur reached her first final in eight months with a comeback 2-6, 6-3, 6-2 win over fourth-seeded Serbian Ana Ivanovic in the Kremlin Cup semi-finals on Saturday.
Top seed Samantha Stosur survived a scare against Alize Cornet before beating the Frenchwoman 7-6, 7-5 to reach the Kremlin Cup quarter-finals on Thursday.
Defending champion Agnieszka Radwanska pulverised Angelique Kerber 6-1, 6-1 to set up a final with Nadia Petrova at the Pan Pacific Open on Friday.
Olympic champion Serena Williams suffered her first loss in nearly three months on Friday as she was knocked out of the Cincinnati Open quarter-finals, falling 6-4, 6-4 to Germany's Angelique Kerber.
Former world number one Serena Williams suffered an injury scare less than two weeks ahead of the Australian Open on Wednesday when the American was forced out of the Brisbane International with a badly twisted ankle.
Samantha Stosur became the highest seeded casualty so far at this year's Wimbledon when she suffered a 6-2, 0-6, 6-4 second round defeat by Dutch player Arantxa Rus to end Australia's interest in the singles.
Images from Day 1 of the French Open 2012 tennis tournament at Roland Garros in Paris, on Sunday.
World number six Samantha Stosur has opted out of Australia's Fed Cup World Group playoff against Ukraine to concentrate on preparing for the clay-court season.
Maria Sharapova marched into the semi-finals of the Cincinnati Open after crushing Australian Samantha Stosur 6-3, 6-2 on Friday to set up a last four meeting with fellow-Russian Vera Zvonareva.
The Cincinnati Open claimed two more high-profile victims on Thursday with Grand Slam champions Li Na and Petra Kvitova crashing out, while Russian pair Maria Sharapova and Vera Zvonareva crunched their opponents en route to the quarter-finals.
Former world number one Serena Williams has pulled out of the Cincinnati Open with a toe injury, tournament organisers said on Wednesday.
The former world number one, who returned to competition in June after 11 months out with injuries and health problems, will carry a 10-match winning streak into the final against Samantha Stosur and appears in ominous form ahead of the US Open starting Aug. 29.
Samantha Stosur's win over world number one Caroline Wozniacki was not the only surprise for an unsuspecting crowd at the WTA Championships, where music, dance and even a frightened feline vied with the tennis for attention.
Francesca Schiavone set up a repeat of last year's French Open final against Samantha Stosur in the Italian Open quarter-finals as top seed Caroline Wozniacki also advanced to the last eight on Thursday.
French second seed Marion Bartoli enjoyed two match wins in one day, including a straight-sets defeat of US Open champion Samantha Stosur, to take the Japan Open title on Sunday.
Barely a month after considering quitting the sport, Russian Dinara Safina celebrated being back in the winner's circle at the Indian Wells WTA tournament on Monday.
The star pull-outs from this year's Commonwealth Games continue with French Open finalist Samantha Stosur becoming the latest to decide against competing in the Delhi event to focus on top WTA tournaments. The Australian tennis ace joins the country's top male tennis player Lleyton Hewitt in pulling out of the Games, according to an Australian Associated Press report.