Indian veteran Leander Paes and his partner Samantha Stosur suffered a second round defeat in the mixed doubles, ending India's campaign at the Australian Open, in Melbourne, on Tuesday.
The unseeded pair won the first set 6-4 against Anna-Lena Groenefeld and Robert Farah but conceded the second by the same scoreline.
The third set went into the tie-break, which the fifth seed German-Colombian duo won 10-8.
Paes and Stosur had beaten the Dutch-Czech pairing of Wesley Koolhof Kveta Peschke in the Round of 32.
The 45-year-old Paes made a first round exit in the men's doubles with Mexican partner Miguel Angel Reyes-Varela.
Jeevan Nedunchezhiyan and partner Nicholas Monroe were also knocked out in the opening round of the event.
In the men's singles, Prajnesh Gunneswaran also made a first round exit after coming through the qualifiers, while Ramkumar Ramanathan, Ankita Raina and Karman Thandi could not make it to the singles main draw.