Bianca Andreescu is the first Canadian to win a major in the professional era.
Biles posted the highest scores of the night on vault (15.800), floor (14.650) and beam (14.350).
The 15-year-old American phenom was forced to dig deep to become the youngest player since Anna Kournikova in 1996 to reach the third round
Serena Williams was the runner-up in Flushing Meadows each of the past two years
Facing the prospect of empty stands at the first fanless Grand Slam since the coronavirus outbreak, top contenders at this year's US Open admit that the unprecedented new hush will take some getting used to.
Serena Williams has not won a set in any of the four Grand Slam finals she has reached since returning from maternity leave in September 2017.
As the fans head toward Flushing Meadows, tennis stars gear up with ground-breaking styles to rule the courts.
Nadal and Williams headline party for all ages at US Open
Bianca Andreescu, 19, the first Canadian to win a major in the professional era, prevented Serena Williams from equalling Margaret Court's record of 24 Grand Slam singles titles.
Images from Day 6 of the US Open in New York, on Saturday.
Images from the second day at the US Open, the final Grand Slam tournament of the year.
Summary of sports events and persons who made news on Tuesday
Serena Williams moved another step closer to a record-tying 24th career Grand Slam title with a comfortable 6-3, 6-2 victory over Czech Karolina Muchova on Friday to reach the last 16 at the US Open.