Dayana Yastremska upset of twice former champion and 18th seed Victoria Azarenka.
The 30-year-old Russian, who returned last month from a 15-month doping ban, said on her website she would play in the qualifying event staged the week before.
Former Wimbledon runner-up Eugenie Bouchard successfully negotiated the qualifying draw on Thursday, beating Colombian Mariana Duque-Marino 6-3, 6-2 to secure a spot in next week's main draw.
American Sam Querry became the latest Wimbledon giant slayer on Saturday as he beat defending champion Novak Djokovic 7-6(6) 6-1 3-6 7-6(5) in the third round. The defeat ended Djokovic's run of winning 30 consecutive grand slam matches. The world number one had arrived at the All England Club as the reigning champion at all four grand slam tournaments.
After a long battle with cancer, Jana died peacefully, surrounded by her family in her native Czech Republic
Anderson, a member of the ATP Player Council, said he felt it was a matter of time before the final-set tiebreak came in.
Stan Wawrinka rolled back the years as the 38-year-old Swiss proved the old adage that class is permanent and form temporary by knocking out seeded Argentine Tomas Etcheverry.
Novak Djokovic said winning a record 23rd men's singles Grand Slam title at the French Open was special given that it was always the toughest one to win for him.
The 36-year-old has been practising hard on the grass and few would bet against her becoming the first mother to win the title since Evonne Cawley 38 years ago.