Simone Biles surpassed Belarusian Vitaly Scherbo's record of 33 medals in total at the Olympics and World Championships.
The 22-year-old's fourth gold medal of the week in Stuttgart, in the balance beam final, ensures she has now overtaken Belarusian Vitaly Scherbo's tally of 23.
Olympic champion Simone Biles became the all-time leading world gymnastics championship gold medal winner when the American claimed her third gold at the Doha championships on Friday.
American Simone Biles became the first woman to win five all-around titles at the gymnastics world championships in Stuttgart.
The four-time Olympic champion is now the owner of 19 gold medals across four championships against 12 for Belarusian Vitaly Scherbo, who competed in five World events between 1991 and 1996.
Simone Biles claimed a record-equalling 23rd medal at the gymnastics world championships on Saturday, leading an American one-two finish in the vault final in Stuttgart.
Simone Biles's shot at a record five gold medals at the Tokyo Olympics suffered a blow on Sunday when the US gymnast failed to qualify for the final on the asymmetric bars.
22-year-old Simone Biles collected a record-extending 15th gold medal at the Gymnastics World Championships.