Biles posted the highest scores of the night on vault (15.800), floor (14.650) and beam (14.350).
Tokyo Games will be the second Olympics for Sanne and Lieke Wevers of the Netherlands.
Disgraced Olympic swimmer Ryan Lochte is joining the 'Dancing With the Stars' cast, ABC television announced on Tuesday, as the gold medalist said he thought reaction to his tale about being held up at a Rio de Janeiro gas station had been blown out of proportion.
'Dancing with the Stars' host Tom Bergeron said on Tuesday he was shocked and unnerved by protesters who shouted "liar, liar" and rushed the stage after a live performance by Ryan Lochte, the gold-medal US swimmer who made up a story about being robbed at the Rio Olympics.
Simone Biles's all five-gold medal Rio Olympic dream is dashed on the balance beam after Wevers upstages her to win beam gold.
The US women's gymnastics team, anchored by all around world champion Simone Biles, won the team gold medal at the Olympics on Tuesday by the widest margin ever.
China continued to put up dominating performances in the pool, this time taking gold in women's 10 metre synchronized diving on Tuesday. Slovenian Judoka Tina Trstenjak became her country's second Olympic champion in the sport.
Simone Biles showed the world why she is an Olympic champion-in-waiting as she and her American teammates blew away the opposition by almost 10 points in women's gymnastics qualifying at the Rio Games on Sunday.
Bahrain's Ruth Jebet blew away the competition to win the women's 3,000 metres steeplechase gold on Sunday, but narrowly missed out on the world record. Jebet ran a bold race, bursting into the lead after a few laps and setting a blistering pace to win in 8 minutes, 59.75 seconds, just shy of the 8:58.81 world record set by Russia's Gulnara Galkina at the 2008 Beijing Games.