Spending by Premier League teams has already broken last season's record and could exceed one billion pounds ($1.32 billion) by the time the transfer window closes at the end of the month.
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Barcelona were third on 648.3 million euros.
Real, Barca topple United off perch to become top earning clubs
Records tumbled in the transfer window as Premier League spending totalled 1.165 billion pounds ($1.54 billion), financial analyst Deloitte's Sports Business Group said on Thursday. The outlay represents a 34 percent rise on the previous highest in all windows of 870 million pounds set 12 months ago and means the overall spend by clubs in 2016-17 already represents a record for a single Premier League season.
Premier League clubs enjoyed record revenues of 3.3 billion pounds ($4.76 billion) in 2014-15 thanks to broadcast deals that will be even more lucrative next season, according to Deloitte.
English football clubs splurged on the final day of the transfer window, laying out 210 million pounds to take total gross spending to a record-high 1.4 billion pounds as the Premier League again flexed its unrivalled financial muscles.
Manchester United have deposed Real Madrid as the world's richest soccer club after revenue grew to a record $735 million in 2015-16, according to Deloitte's annual Money League published on Thursday.
English clubs took their spending on players for the season soaring past 1 billion pounds on Monday, but the biggest signing on transfer deadline day was that of a manager as Manchester City confirmed Pep Guardiola will take over this summer.