Inter Milan were confirmed as 2006 Serie A champions on Monday after Italy's football federation (FIGC) said it did not have the authority to revoke the decision which stripped Juventus of the title.
"The federal council has turned down Juventus' petition regarding the 2006 scudetto with a declaration that it is not competent to revoke it," the FIGC said in a statement.
Inter, who were handed the 2006 title by default after table-toppers Juventus were demoted and second-placed AC Milan docked points for influencing the selection of referees, had been under investigation since last year after new evidence emerged in the criminal case into the match-fixing scandal.
Lawyers said phone taps also implicated Inter in the affair for the first time but the club denied wrongdoing.
Juventus reacted to Monday's decision by threatening to seek "justice" in other courts, stating the ruling was "a long way from re-establishing fairness".
"The club has given the go ahead for its lawyers to explore the best means of proceeding in international and administrative law," the club said in a statement on its website (www.juventus.com).
"At the same time the management and its lawyers are evaluating the economical damages such behaviour has caused."
FIGC president Giancarlo Abete said Juventus were free to take their case to other bodies.
"I don't believe taking it to the courts would be the best choice but I'd respect the decision," said Abete.
"The credibility of the system is tied to respect for the rules."