It was learnt that 35 registered players, including seven foreigners, wrote to the club that they didn't wish to go to Iran.
The Champions League Two features 32 clubs divided into eight groups - four West, four East - of four teams each for the group stage, which will be played in a home-and-away round-robin format from September 17 to December 5.
Keeping their players' "safety and security" in mind, Mohun Bagan Super Giant have decided against travelling to Iran for their AFC Champions League 2 match against Tractor FC owing to the existing volatile situation in the west Asian nation.