The fast bowler is down with back spasms and likely to miss the first Test between India and England.
Flintoff, who has twice undergone operations on his ankle, reported some discomfort after playing for his county Lancashire against Hampshire on Saturday.
The two players will join England's injury-hit touring party ahead of the first Test against India.
The England captain will sit out of the three-day tour match against Board President's XI due to recurrence of a knee injury
Simon Jones Shaun Udal, Ian Blackwell and Monty Panesar are ill with a stomach bug.
The England selectors have appointed Trescothick as captain for the first Test in Vaughan's absence.
Injury-hit England have called up Middlesex batsman Owais Shah as cover for their test series in India starting on Wednesday, a team official said.
The Board of Control for Cricket in India wants the first Test against England at Ahmedabad put off by a day to allow Mahendra Singh Dhoni play in the Champions T20 League final two days prior to it in case his team, Chennai Super Kings, makes it to the title-contest. The Test opener is to be held from December 11 at Ahmedabad's Motera stadium a day after the Champions League final at Chennai.
The first match in England's controversial tour of Zimbabwe has been rescheduled for Sunday.
The England opener would be returning home to be with his wife, who is pregnant and close to delivery
England's five-match cricket tour of Zimbabwe will go ahead after a ban on 13 British journalists was rescinded.
Giles will undergo surgery in a bid to make a complete recovery before England's tour of India early next year.
The Indian team boosts ECB revenues. No wonder that the ECB wants India to come as often as possible and for full five-Test series, regardless of their poor showing, reports Ashis Ray.