Agassi said in a statement released by the organisers that it was a regrettable decision and he would miss playing in the tournament.

The exact nature of his injury was not disclosed.

Also Read Federer stalks triple crown on grass

Agassi, 35, who is ranked sixth in the world, won his first grand slam title when he defeated Croatian Goran Ivanisevic in a five-set Wimbledon final in 1992.

The American has since won four Australian Opens, two U.S. Opens and one French Open.

Women's world number 14 Elena Bovina of Russia has also withdrawn from Wimbledon because of a shoulder injury.