The pacer was given painkillers and was ready to bat at number 11 if needed during England's chase, which ended with seven balls to still go due to rain.
With Morgan, England's T20 vice-captain, too ruled out due to a shoulder injury there is no obvious replacement for Broad as captain.
Another cause for concern is that Broad could also miss the five-match ODI tour of India next month.
Broad bowled well at Lord's, picking up the wickets of openers Parthiv Patel and Ajinkya Rahane in his first two overs.
It has been an injury-hit last 10 months for Broad, who was ruled out of the final three Ashes Tests last winter due to stomach strain, before picking up a rib injury during the World Cup that forced him to return home.
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